ST-E00211:326:H5L3NCCXY:2:2219:23328:63173 83 chr14 21853647 24 141M = 21853647 -141 ACTTCACCTCCTGGAGTCCTGGACTTCCCCACATCTCCCCTGCCCCTCCCACGTTTCCATAGTCCAAGGGCCAGAGTAAATGAAAATACAGCAGCCGCCCAAGCAATGGGGCCCATGCTGGGGCTTCAGTCATCCCCATGT JFJFJFJJJJJJJJJJJJJJJFAJJJJJJJJJFFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFFFAA AS:i:-28 XN:i:0 XM:i:5 XO:i:0 XG:i:0 NM:i:5 MD:Z:0T0T132A0G3C1 YS:i:-27 YT:Z:CP ST-E00211:326:H5L3NCCXY:2:2219:23328:63173 163 chr14 21853647 24 141M = 21853647 -141 ACTTCACCTCCTGGAGTCCTGGACTTCCCCACATCTCCCCTGCCCCTCCCACGTTTCCATAGTCCAAGGGCCAGAGTAAATGAAAATACAGCAGCCGCCCAAGCAATGGGGCCCATGCTGGGGCTTCAGTCATCCCCATGT A<FFFFFFFJJFJFJJ<<JJJJJJJJJJJJFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJJFJJJ<JFJJJJJJJJAJJFJJJJJJJJJJJJJJJJJFJJJJFJJJJJJJJJJAJJJJJJJJJJJJJJJJ AS:i:-27 XN:i:0 XM:i:5 XO:i:0 XG:i:0 NM:i:5 MD:Z:0T0T132A0G3C1 YS:i:-28 YT:Z:CP
这是双端测序的sam文件随意挑出的一条测序片段。（sam文件会把同一片段的两条read都保留，从第二列可以知道是read1还是read2），可以发现这条片段已经被测通（因为染色体坐标一致），基因序列整体是相同的，说明read1和read2都是按5‘-->3’的顺序排列的