#!/usr/bin/python import pandas as pd df = pd.read_excel('c:datazichan.xlsx') df_sn = pd.read_excel('c:dataSN.xlsx') sn_series = pd.Series(df_sn['SN'].values) print(sn_series.size) new_df = df[~df['SN'].isin(sn_series)] new_df.to_excel('c:dataout.xlsx')