安装Randfold
软件下载：http://bioinformatics.psb.ugent.be/supplementary_data/erbon/nov2003/
安装：tar zxvf randfold.tar.gz
cd randfold-2.0
make
cp randfold /usr/local/bin
安装前需安装Sean Eddy's SQUID library
软件下载：http://selab.janelia.org/software.html
安装：tar zxvf squid-1.9g.tar.gz
./configure
make
make install
关于randfold
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RANDFOLD - A randsomization test for sequence secondary structure
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This is RANDFOLD version 2. The software compute the probability that, for a given sequence, the Minimum Free Energy (MFE) of the secondary structure is different from MFE computed with random sequences.
For more information, see the paper published in Bioinformatics:
Bonnet E, Wuyts J, Rouze P, Van de Peer Y. Evidence that microRNA precursors, unlike other non-coding RNAs, have lower folding free energies than random sequences.
Bioinformatics. 2004 Nov 22;20(17):2911-7.
PMID: 15217813