Audrey presents our recent work at the Nanopore Community Meeting 2019.
Our genome has different levels of organization, and at the nucleosome level, DNA strands wrap around protein cores like beads on a string. Positioning of these nucleosomes and changes in chromatin status play important regulatory roles in gene expression. Our new method, MeSMLR-seq, aims to resolve the long-range dynamics that remain poorly understood at the single-molecule level.
Briefly, two examples from the paper in Genome Research are discussed:
1) MeSMLR-seq can resolve differential nucleosome organization principles at the transcription start site of silent versus active genes; and
2) MeSMLR-seq can explore the coupled chromatin status of adjacent genes during transcriptional reprogramming.
These methods offer promising utility to study multiple layers of epigenetics simultaneously.
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我们的基因组有不同层次的组织，在核小体层次上，DNA链像串珠一样缠绕在蛋白质核心上。
这些核小体的定位和染色质状态的改变在基因表达中起着重要的调控作用。
我们的新方法，MeSMLR-seq，旨在解决在单分子水平上仍未被充分理解的远距离动力学。
简要地讨论一下这篇论文在基因组研究中的两个例子:
1) MeSMLR-seq可以解决沉默基因和活性基因转录起始位点不同的核小体组织原则;
和
2) MeSMLR-seq可以探索转录重编程过程中相邻基因的偶联染色质状态。
这些方法为同时研究表观遗传学的多个层面提供了前景。